منابع مشابه
Recombinant nucleases CEL I from celery and SP I from spinach for mutation detection
BACKGROUND The detection of unknown mutations is important in research and medicine. For this purpose, a mismatch-specific endonuclease CEL I from celery has been established as a useful tool in high throughput projects. Previously, CEL I-like activities were described only in a variety of plants and could not be expressed in an active form in bacteria. RESULTS We describe expression of activ...
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BACKGROUND The enzymatic mutation detection (EMD) assay uses the bacteriophage resolvase T4 endonuclease VII, which cleaves preformed heteroduplex molecules at mismatch sites, forming two shorter fragments that can be resolved by gel electrophoresis. The method can be used to detect single and multiple base changes, as well as insertions and deletions. METHODS The sensitivity, specificity, an...
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Yeast-based assays have been developed to detect inactivating mutations in human genes, but these assays generally rely on the human protein having a biological function in yeast. We describe a simple method to detect mutations by virtue of their ability to abolish a protein-protein interaction in the yeast two-hybrid assay. By the use of direct recombinational cloning in yeast of a reverse tra...
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The Enzymatic Mutation Detection (EMD) assay detects mutations or polymorphisms in DNA. The assay procedure takes <1 h and is followed by electrophoretic detection. We report an automated procedure, using fluorescently labeled probe and quantitative analysis on the ABI Prism 377 DNA Sequencer, that improves on earlier methods (1, 2) by eliminating the need for sample purification, shortening th...
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The polyamine spermidine (NH,(CH&NH(CH&NH,) is widespread in nature. It is present in mammalian tissues (1, 2), plants (3), bacteria (47), ribosomes (6, 8), and bacteriophages (9-11). There are various methods for its identification, including steam distillation (12)) paper chromatography (13-18)) paper electrophoresis (14, 16, 17, 19), ion exchange chromatography (2, 5), gas chromatography (31...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2000
ISSN: 0736-6205,1940-9818
DOI: 10.2144/00291bm07